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Laboratory
Methods for Confirmation of Orthopoxviruses
There
are a number of methods for confirming a case involving orthopoxvirus.
PCR-related methods for DNA Identification can be used. PCR tests
include Real-time PCR, Single-gene PCR/RFLP or Pan-genomic methods,
if indicated. Electron microscopy, histopathology, culture and
serology are other methods used for identifying orthopoxviruses.
Serologic methods may help to evaluate the extent of an immune
response, but will not be helpful to determine if replicative vaccinia
is the cause of a vaccine associated adverse event.
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