Laboratory Support

Laboratory Methods for Confirmation of Orthopoxviruses

There are a number of methods for confirming a case involving orthopoxvirus. PCR-related methods for DNA Identification can be used. PCR tests include Real-time PCR, Single-gene PCR/RFLP or Pan-genomic methods, if indicated. Electron microscopy, histopathology, culture and serology are other methods used for identifying orthopoxviruses. Serologic methods may help to evaluate the extent of an immune response, but will not be helpful to determine if replicative vaccinia is the cause of a vaccine associated adverse event.

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