Browsing by Author "Min, Sang-Won"
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Item Studies on Plasma Membrane Proteins Involved in Membrane Traffic: Syntaxins and E-Syts(2007-05-22) Min, Sang-Won; Südhof, Thomas C.Fusion of synaptic vesicles is catalyzed by SNARE complex assembly which requires that the SNARE proteins syntaxin-1A and -1B, two isoforms of syntaxin-1, switch from a 'closed' to an 'open' conformation. To test the physiological significance of this switch, I analyzed mutant mice with a point mutation in syntaxin-1B which renders it predominantly 'open' in the syntaxin-1A null background. Whereas deletion of syntaxin-1A caused no detectable phenotype, opening of syntaxin-1B produced lethal epilepsy, independent of the presence of syntaxin-1A. Morphological and electrophysiological analyses revealed that opening of syntaxin-1B impaired steps in exocytosis upstream of vesicle priming, but enhanced Ca2+