Browsing by Subject "Kidney"
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Item Acute kidney injury--forging ahead!(2009-02-20) Dev, Devasmita C.Item Acute kidney injury: the beginning of the end of the dark ages(2010-04-16) Lu, Christopher Y.Item Acute renal failure: ischemia, inflammation, and sepsis(2003-07-24) Lu, Christopher Y.Item An analysis of the renal protection afforded by calcium channel blockers(1990-05-03) Seney, Frank D., Jr.Item Atrial natriuretic factor(1985-08-22) Henrich, William L.Item Beta-Catenin and Development of the Urogenital System(2009-09-04) Merkel, Calli E.; Carroll, Thomas J.The urogenital system is composed of the kidneys, gonads, urinary and reproductive tracts. Components of the urogenital system play many important roles in the body; kidneys function by regulating the body's fluid volume, acidity and mineral composition, while the reproductive tract is necessary for propagation of our species. Therefore, the study of urogenital system development is important in the understanding of disorders associated with both the kidneys and the reproductive tract and their treatment. Urogenital system development begins with the formation of an epithelial tube, called the Wolffian duct. From the Wolffian duct forms a ureteric bud, which, along with the metanephric mesenchyme, will undergo a series of morphogenetic changes, eventually giving rise to the adult kidney. The Wolffian or M��rian ducts, along with the bipotential gonads, will develop into the male or female reproductive tracts,respectively. Although many signals are involved in development of the urogenital system, canonical Wnt/beta-catenin signaling is known to play a significant role. To better understand the role Wnt signaling plays in reproductive tract development, we conditionally removed beta-catenin from the Wolffian duct using a HoxB7cre line of mice. We determined that beta-catenin is necessary for M��rian duct formation. Additionally, removal of beta-catenin from the Wolffian duct leads to premature differentiation, preventing degradation of the Wolffian duct in females and inhibiting proper formation of the Wolffian duct into components of the male reproductive tract. In addition to our mouse model, we validated the efficacy of small molecule inhibitors of Wnt signaling in embryonic kidney culture. Functional small molecule Wnt inhibitors will provide an important tool for the continued study of urogenital system development along with the potential treatment of diseases associated defective Wnt signaling.Item Clinical implications of impaired renal autoregulation(2000-10-12) Palmer, Biff F.Item Investigating Vascular Patterning and Regression in Kidney Development and Organoids(December 2021) Ryan, Anne Regina; Marciano, Denise; Munshi, Nikhil; Dellinger, Michael T.; Cleaver, OndineChronic kidney disease (CKD) and end stage renal disease (ESRD) are increasingly frequent and devastating conditions that have driven a surge in the need for kidney transplantation. A stark shortage of organs has fueled interest in generating viable replacement tissues ex vivo for transplantation. One promising approach has been self-organizing organoids, which mimic developmental processes and yield multicellular, organ-specific tissues. However, a recognized roadblock to this approach is that many organoid cell types fail to acquire full maturity and function. I comprehensively assessed the vasculature in two distinct kidney organoid models as well as in explanted embryonic kidneys. Using a variety of methods, my work shows that while organoids can develop a wide range of kidney cell types, as previously shown, endothelial cells (ECs) initially arise but then rapidly regress overtime in culture. Vasculature of cultured embryonic kidneys exhibit similar regression. By contrast, engraftment of kidney organoids under the kidney capsule results in the formation of a stable, perfused vasculature that integrates into the organoid. This work demonstrates that kidney organoids offer a promising model system to define the complexities of vascular-nephron interactions, but the establishment and maintenance of a vascular network present unique challenges when grown ex vivo. The future of the field necessitates the inclusion of flow and perhaps additional factors into in vitro culture methods. Future studies investigating endothelial heterogeneity in the developing kidney will aid in forwarding our mission of creating a functional organoid vasculature.Item Kidney and aging: more than what you think you know(2010-06-04) Huang, Chou-LongItem The kidney in connective tissue diseases(1962-02-08) Hess, Evelyn V.Item Living kidney transplantation: good for recipient, how about donor?(2008-08-01) Rajora, NilumItem [News](1988-02-12) Rutherford, SusanItem Pharmacologic role of the kidney(1978-11-09) Brater, D. CraigItem Physiology and pathophysiology of the aging kidney(1986-01-16) Levi, MosheItem Progression of chronic kidney disease: diagnosis, management and prevention(2007-05-25) Toto, Robert D.Item Renal acidosis(1967-04-13) Wadi, SukiItem Item Renal transplantation 1984; in celebration of 20 years of transplantation at PMH-SWMS(1984-10-25) Helderman, J. HaroldItem The role of the kidney in congestive heart failure(1985-10-10) Kokko, Juha P.