Browsing by Subject "Sarcoma, Kaposi"
Now showing 1 - 4 of 4
- Results Per Page
- Sort Options
Item Acquired immunodeficiency syndrome(1984-09-06) Lipsky, Peter E.Item HIV associated Kaposi sarcoma: the case for an interdisciplinary approach(2022-03-04) Jones, Amy; Knights, Sheena; Lau, Abby; Lazarte, SusanaItem Kaposi's sarcoma: an opportunistic neoplasm?(1985-03-07) Tigelaar, Robert E.Item The PNUTS-PP1 Complex Acts as an Intrinsic Barrier to Kaposi's Sarcoma Associated Herpesvirus Gene Expression and Replication(2023-05-01T05:00:00.000Z) Devlin, Anne Marie; D'Orso, Iván; Reese, Tiffany A.; Kraus, W. Lee; Conrad, NicholasControl of RNA Polymerase II (pol II) elongation is a critical component of gene expression in mammalian cells. The PNUTS-PP1 complex controls elongation rates, slowing pol II after polyadenylation sites to promote termination. The Kaposi's sarcoma-associated herpesvirus (KSHV) co-opts pol II to express its genes, but little is known about its regulation of pol II elongation. I identified PNUTS as a suppressor of a KSHV reporter gene in a genome-wide CRISPR screen. PNUTS depletion enhances global KSHV gene expression and overall viral replication. Mechanistically, PNUTS requires PP1 interaction, binds viral RNAs downstream of polyadenylation sites, and restricts transcription readthrough of viral genes. Surprisingly, PNUTS also represses productive elongation at the 5´ ends of the KSHV reporter and the KSHV T1.4 RNA. From these data, I conclude that PNUTS' activity constitutes an intrinsic barrier to KSHV replication likely by suppressing pol II elongation at promoter-proximal regions.