Role of KIRREL in Mammalian Myogenesis and Rhabdomyosarcoma
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Rhabdomyosarcoma is an aggressive soft-tissue malignancy comprised microscopically of neoplastic skeletal muscle-lineage precursors that fail to exit the cell-cycle and fuse into syncytial muscle - the underlying pathogenetic mechanisms for which remain unclear. We previously identified that misregulated myoblast fusion signaling via the TANC1 adaptor molecule promotes neoplastic transformation in RMS cells driven by the PAX-FOXO1 oncogenic transcription factor. Unknown from these studies are the upstream elements that participate with TANC1 in wild-type myoblasts to orchestrate myoblast fusion, and how these elements likewise participate in RMS. We are now interrogating the Immunoglobulin Superfamily Receptor Kirrel receptors in these processes. Three Kirrel orthologs are present in mammals, two of which, Kirrel-1 and -3, are expressed normally in myogenesis. Using loss-of-function (shRNA) and gain-of-function (misexpression) strategies, our preliminary data are showing a requirement for Kirrel-3 during myoblast fusion, as tube formation is significantly altered in these studies. We are now extending these for Kirrel-3 activity in RMS using cultured neoplastic rhabdomyoblast. Our data and most recent findings will be presented and discussed.
The 56th Annual Medical Student Research Forum at UT Southwestern Medical Center (Tuesday, January 23, 2018, 2-5 p.m., D1.600)
Each year the Medical Student Research Program awards students for the best oral presentation and the best poster presentation as judged by faculty across campus. This author received an award as one of the best poster presentations at this forum.
SubjectBasic Research and Disease Models
Best Poster Presentation Award
Reinert, S., Avirneni, U., & Galindo, R. (2018, January 23). Role of KIRREL in mammalian myogenesis and rhabdomyosarcoma. Poster session presented at the 56th Annual Medical Student Research Forum, Dallas, TX. Retrieved from https://hdl.handle.net/2152.5/5325