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dc.contributor.advisorSherry, A. Deanen
dc.creatorRussell, C. Hunter, IIIen
dc.date.accessioned2010-07-12T18:54:24Z
dc.date.available2010-07-12T18:54:24Z
dc.date.issued2004-05-04
dc.identifier.urihttps://hdl.handle.net/2152.5/716
dc.description.abstractA method for delivering Gd-based MRI contrast agents by ultrasound-targeted microbubble destruction into fibroblast cells in vitro is presented. First, MRI contrast agents Gd-F-DOTP-ME and Gd-DOTA-4iBA are shown to associate with liposome microbubbles used as ultrasound contrast agents. Repeated washings with phosphate buffered saline followed by T1 measurements at 20 MHz revealed Gd-DOTA-4iBA was retained more effectively by the liposome microbubbles. Secondly, ultrasound-targeted microbubble destruction using Optison(tm) microbubbles to deliver Gd-DOTA-4iBA into fibroblast cells displays negligible T1 shortening at 4.7T over controls. Further experiments are planned to validate these observations with other MRI contrast agents and other forms of microbubbles used for ultrasound image enhancement. The goal of these experiments is eventually combining MRI contrast agent delivery with ultrasound targeted microbubble destruction gene therapy to quantitatively determine the amount of DNA delivered into target tissues.en
dc.format.mediumElectronicen
dc.format.mimetypeapplication/pdfen
dc.language.isoenen
dc.subjectMicrobubblesen
dc.subjectMagnetic Resonance Imagingen
dc.subjectMyofibroblasten
dc.titleThe Use of Ultrasound-Targeted Microbubble Destruction in Mediated Delivery of a Gadolinium Magnetic Resonance Imaging Contrast Agent Into Fibroblast Cells In Vitroen
dc.typeThesisen
dc.type.materialTexten
dc.type.genredissertationen
dc.format.digitalOriginborn digitalen
thesis.degree.grantorUT Southwestern Medical Centeren
thesis.degree.departmentGraduate School of Biomedical Sciencesen
thesis.degree.nameMaster of Scienceen
thesis.degree.levelMastersen
thesis.degree.disciplineBiomedical Engineeringen
thesis.date.available2004-05-04
dc.identifier.oclc58995398


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