The Mitotic Spindle Mediates Inheritance of the Golgi Ribbon Structures

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dc.contributor.advisorSeemann, Joachimen
dc.creatorWei, Jen-Hsuanen
dc.date.accessioned2010-07-12T18:56:39Z
dc.date.available2010-07-12T18:56:39Z
dc.date.issued2010-05-14
dc.description.abstractThe mammalian Golgi ribbon disassembles during mitosis and reforms in both daughter cells after division. Mitotic Golgi membranes concentrate around the spindle poles, suggesting that the spindle may control Golgi partitioning. To test this, cells were induced to divide asymmetrically with the entire spindle segregated into only one daughter cell. A ribbon reforms in the nucleated karyoplasts, whereas the Golgi stacks in the cytoplasts are scattered. However, the scattered Golgi stacks are polarized and transport cargo. Microinjection of Golgi extract together with tubulin or incorporation of spindle materials rescues Golgi ribbon formation. Therefore, the factors required for postmitotic Golgi ribbon assembly are transferred by the spindle, but the constituents of functional stacks are partitioned independently, suggesting that Golgi inheritance is regulated by two distinct mechanisms.en
dc.format.digitalOriginborn digitalen
dc.format.mediumElectronicen
dc.format.mimetypeapplication/pdfen
dc.identifier.oclc795519922
dc.identifier.urihttps://hdl.handle.net/2152.5/732
dc.language.isoenen
dc.subjectMitotic Spindle Apparatusen
dc.subjectGolgi Apparatusen
dc.subjectMitosisen
dc.titleThe Mitotic Spindle Mediates Inheritance of the Golgi Ribbon Structuresen
dc.typeThesisen
dc.type.genredissertationen
dc.type.materialTexten
thesis.date.available2012-05-14
thesis.degree.departmentGraduate School of Biomedical Sciencesen
thesis.degree.disciplineCell Regulationen
thesis.degree.grantorUT Southwestern Medical Centeren
thesis.degree.levelDoctoralen
thesis.degree.nameDoctor of Philosophyen

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