Myokine Musclin Expression Is Elevated in Rats after Burn

dc.contributor.otherSong, Juquanen
dc.contributor.otherSehat, Alvanden
dc.contributor.otherMaxwell, Christianen
dc.contributor.otherPanwar, Kunalen
dc.contributor.otherKulangara, Rohanen
dc.contributor.otherCarlson, Deborahen
dc.contributor.otherHuebinger, Ryanen
dc.contributor.otherWolf, Stevenen
dc.creatorMaredia, Navinen
dc.creator.orcid0000-0002-3315-0684
dc.date.accessioned2018-07-25T15:39:25Z
dc.date.available2018-07-25T15:39:25Z
dc.date.issued2017-01-17
dc.descriptionThe 55th Annual Medical Student Research Forum at UT Southwestern Medical Center (Monday, January 17, 2017, 2-5 p.m., D1.600)en
dc.description.abstractINTRODUCTION: Annually, over 2 million people in the US experience severe burns, a condition marked by a hypercatabolic state with significant muscle loss. Muscle is necessary for glucose and lipid metabolism. Previous studies have shown detrimental effects of insulin resistance and hyperglycemia associated with muscle loss due to burns. Recently, the novel skeletal muscle myokine musclin has been found to regulate glucose in vitro. Thus, we attempted to better understand the effects of burns on musclin levels. We aimed to investigate the effects of burns on 1.) musclin levels systemically and 2.) musclin mRNA expression in vitro. METHODS: Thirty-one adult male Sprague-Dawley rats received 40% total body surface area (TBSA) burns. Rat serum was collected from 6 hours to 14 days after burn. Nine animals without injury served as control. Musclin levels in serum were measured by ELISA. Mouse C2C12 myoblasts were stimulated with 10% rat burn serum for 24 hours. Cells were stimulated with non-burn serum, 6-hour post burn serum, 72-hour post burn serum, and 14-day post burn serum. Following stimulation for 24 hours, C2C12 cells were collected and musclin expression was quantified by real time PCR analysis. RESULTS: Circulating musclin levels were 59.3 ± 3.3 ng/mL in non burned control rats. Musclin levels in the serum significantly increased to 76.7 ± 6.0 ng/mL at 6 hours and 76.7 ± 1.9 ng/mL at 24 hours after burn (p<0.05). Musclin levels in the serum returned to baseline until 14 days. Normalized to GAPDH mRNA level, musclin mRNA expression was 7.87 ± 0.56 fold in C2C12 myoblasts with 10% non-burn serum stimulation. Musclin mRNA expression significantly increased with the addition of the following burn rat serums: 22.66 ± 5.18 fold with 6-hour post-burn serum, 15.00 ± 1.93 fold with 72-hour post-burn serum, and 12.17 ± 0.82 fold with 14-day post-burn serum (p<0.05). CONCLUSIONS: Musclin levels increase in rat serum following 40% TBSA burn injury. In vitro stimulation of muscle cells with burn serum increases musclin expression.en
dc.description.sponsorshipSouthwestern Medical Foundationen
dc.identifier.citationMaredia, N., Song, J., Sehat, A., Maxwell, C., Panwar, K., Kulangara, R., . . . Wolf, S. (2017, January 17). Myokine musclin expression is elevated in rats after burn. Poster session presented at the 55th Annual Medical Student Research Forum, Dallas, TX. Retrieved from https://hdl.handle.net/2152.5/5548en
dc.identifier.urihttps://hdl.handle.net/2152.5/5548
dc.language.isoenen
dc.relation.ispartofseries55th Annual Medical Student Research Forumen
dc.subjectBasic Research and Disease Modelsen
dc.subject.meshBurnsen
dc.subject.meshMuscle Proteinsen
dc.subject.meshMuscle, Skeletalen
dc.titleMyokine Musclin Expression Is Elevated in Rats after Burnen
dc.typePresentationen

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