Understanding RNA Regulation Through Analysis of CLIP-Seq Data

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dc.contributor.advisorMendell, Joshua T.en
dc.contributor.committeeMemberXie, Yangen
dc.contributor.committeeMemberXiao, Guanghuaen
dc.contributor.committeeMemberMangelsdorf, David J.en
dc.contributor.committeeMemberZhang, Michael Q.en
dc.creatorWang, Taoen
dc.date.accessioned2018-01-04T21:11:15Z
dc.date.available2018-01-04T21:11:15Z
dc.date.created2015-12
dc.date.issued2015-11-18
dc.date.submittedDecember 2015
dc.date.updated2018-01-04T21:04:48Z
dc.description.abstractThe past decades have witnessed a surge of discoveries revealing RNA regulation as a central player in cellular processes. The advent of cross-linking immunoprecipitation coupled with high-throughput sequencing (CLIP-Seq) technology has recently enabled the investigation of genome-wide RNA binding protein-RNA interactions, which is a very important component of RNA-regulation. However, proper and systematic bioinformatics analysis of CLIP-Seq data is still lacking and challenging. For the past few years, I have been devoting my research to methodological developments of CLIP-Seq data analysis, and developed MiClip and dCLIP for peak calling and differential analysis of CLLIP-Seq data, respectively. I have also applied my CLIP-Seq analysis pipelines in on-campus collaborating projects, in which I identified ORF57 and nuclear AGO2 binding sites. Finally, I conducted analysis of public CLIP-Seq datasets to systematically characterize RNA binding protein targeting sites on circular RNAs.en
dc.format.mimetypeapplication/pdfen
dc.identifier.oclc1017760201
dc.identifier.urihttps://hdl.handle.net/2152.5/4457
dc.language.isoenen
dc.subjectGenomicsen
dc.subjectHigh-Throughput Nucleotide Sequencingen
dc.subjectRNAen
dc.subjectRNA-Binding Proteinsen
dc.subjectSequence Analysis, RNAen
dc.titleUnderstanding RNA Regulation Through Analysis of CLIP-Seq Dataen
dc.typeThesisen
dc.type.materialtexten
thesis.degree.departmentGraduate School of Biomedical Sciencesen
thesis.degree.disciplineIntegrative Biologyen
thesis.degree.grantorUT Southwestern Medical Centeren
thesis.degree.levelDoctoralen
thesis.degree.nameDoctor of Philosophyen

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