Pax7 Expression Defines Germline Stem Cells in the Adult Testis

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dc.contributor.advisorAmatruda, James F.en
dc.contributor.committeeMemberCastrillon, Diego H.en
dc.contributor.committeeMemberZinn, Andrew R.en
dc.contributor.committeeMemberHamra, F. Kenten
dc.creatorAloisio, Ginaen
dc.date.accessioned2019-06-03T19:51:25Z
dc.date.available2019-06-03T19:51:25Z
dc.date.created2017-05
dc.date.issued2015-06-09
dc.date.submittedMay 2017
dc.date.updated2019-06-03T19:51:26Z
dc.descriptionThe file named "ALOISIO-DISSERTATION-2017.pdf" is the primary dissertation file. Four (4) supplemental files are also available and may be viewed individually.en
dc.description.abstractSpermatogenesis is a complex, multistep process that maintains male fertility and is sustained by rare germline stem cells. Spermatogenic progression begins with spermatogonia, populations of which express distinct markers. The identity of the spermatogonial stem cell population in the undisturbed testis is controversial due to a lack of reliable and specific markers. Here we identified the transcription factor Pax7 as a specific marker of a rare subpopulation of A(single) spermatogonia in mice. Pax7+ cells were present in the testis at birth. Compared with the adult testis, Pax7+ cells constituted a much higher percentage of neonatal germ cells. Lineage tracing in healthy adult mice revealed that Pax7+ spermatogonia self-maintained and produced expanding clones that gave rise to mature spermatozoa. Interestingly, in mice subjected to chemotherapy and radiotherapy, both of which damage the vast majority of germ cells and can result in sterility, Pax7+ spermatogonia selectively survived, and their subsequent expansion contributed to the recovery of spermatogenesis. Finally, Pax7+ spermatogonia were present in the testes of a diverse set of mammals. Our data indicate that the Pax7+ subset of A(single) spermatogonia functions as robust testis stem cells that maintain fertility in normal spermatogenesis in healthy mice and mediate recovery after severe germline injury, such as occurs after cancer therapy.en
dc.format.mimetypeapplication/pdfen
dc.identifier.oclc1103324520
dc.identifier.urihttps://hdl.handle.net/2152.5/6591
dc.language.isoenen
dc.subjectPAX7 Transcription Factoren
dc.subjectStem Cellsen
dc.subjectTestisen
dc.titlePax7 Expression Defines Germline Stem Cells in the Adult Testisen
dc.typeThesisen
dc.type.materialtexten
thesis.degree.departmentGraduate School of Biomedical Sciencesen
thesis.degree.disciplineCancer Biologyen
thesis.degree.grantorUT Southwestern Medical Centeren
thesis.degree.levelDoctoralen
thesis.degree.nameDoctor of Philosophyen

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UT Southwestern Graduate School of Biomedical Sciences