The Role of the WWTR1(TAZ)-CAMTA1 Gene Fusion in Epithelioid Hemangioendothelioma

dc.contributor.advisorDellinger, Michael T.en
dc.contributor.committeeMemberCleaver, Ondineen
dc.contributor.committeeMemberMcFadden, David G.en
dc.contributor.committeeMemberPan, Duojiaen
dc.creatorDriskill, Jordan Harrisonen
dc.creator.orcid0000-0003-4319-3845
dc.date.accessioned2023-06-07T18:15:19Z
dc.date.available2023-06-07T18:15:19Z
dc.date.created2021-05
dc.date.issued2021-05-01T05:00:00.000Z
dc.date.submittedMay 2021
dc.date.updated2023-06-07T18:15:20Z
dc.description.abstractEpithelioid hemangioendothelioma (EHE) is a devastating and mysterious vascular cancer which has no known definitive treatment. Due to a lack of valid animal or cell-based models of EHE, progress toward understanding and treating this cancer has been severely limited. However, recent studies have determined that 90% of patients exhibit a lone, characteristic in-frame gene fusion, TAZ(WWTR1)-CAMTA1. While expression of the TAZ-CAMTA1 fusion protein has been validated as a biomarker of EHE, it remains unknown whether this genetic abnormality is a passenger or a driver of EHE. In this project, I present the first genetically-engineered mouse model (GEMM) of EHE, showing that the expression of the TAZ-CAMTA1 protein in endothelial cells is sufficient to drive the formation of EHE-like tumors in the lungs of mice. Furthermore, I demonstrate that the cessation of TAZ-CAMTA1 expression leads to the regression of these vascular tumors. I also demonstrate that TAZ-CAMTA1 transforms the MS1 endothelial cell line and that subcutaneous transplantation of these cells into nude mice leads to the formation of solid, progressive EHE-like vascular tumors that have the capacity to metastasize to the lung. Utilizing these two novel models of EHE, I unravel the gene program of TAZ-CAMTA1 and demonstrate that TAZ-CAMTA1 drives a gene signature similar to TAZ, the key effector of the Hippo pathway. Expression of an activated TAZ in endothelial cells is also sufficient to drive EHE-like vascular tumors in mice, and genetic blockade of the transcriptional partners of TAZ, the TEAD family of transcription factors, prevents the formation of TAZ-CAMTA1-induced vascular tumors. Next, I show that TAZ-CAMTA1 induces an angiogenic and regenerative-like gene program in endothelial cells. I validate that TAZ-CAMTA1 exhibits gain-of-function activities by having increased resistance to proteasomal degradation and increased nuclear enrichment over TAZ. Lastly, I show that TAZ-CAMTA1 still maintains its binding to the Hippo pathway proteins which are known to negatively regulate TAZ. In summary, I generate two novel models that pinpoint TAZ-CAMTA1 as the key driver of EHE and utilize these models to suggest several new lines of investigation for the treatment of patients with EHE.en
dc.format.mimetypeapplication/pdfen
dc.identifier.oclc1381370051
dc.identifier.urihttps://hdl.handle.net/2152.5/10055
dc.language.isoenen
dc.subjectCalcium-Binding Proteinsen
dc.subjectCarcinogenesisen
dc.subjectEndothelial Cellsen
dc.subjectGene Expression Regulation, Neoplasticen
dc.subjectHemangioendothelioma, Epithelioiden
dc.subjectIntracellular Signaling Peptides and Proteinsen
dc.subjectTrans-Activatorsen
dc.titleThe Role of the WWTR1(TAZ)-CAMTA1 Gene Fusion in Epithelioid Hemangioendotheliomaen
dc.typeThesisen
dc.type.materialtexten
thesis.degree.departmentGraduate School of Biomedical Sciencesen
thesis.degree.disciplineGenetics, Development and Diseaseen
thesis.degree.grantorUT Southwestern Medical Centeren
thesis.degree.levelDoctoralen
thesis.degree.nameDoctor of Philosophyen

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