Structural Basis of Calcium Transport and Selectivity in the Mitochondrial Calcium Uniporter

Simple item page
dc.contributor.advisorRosenbaum, Daniel M.en
dc.contributor.committeeMemberBlount, Paulen
dc.contributor.committeeMemberHibbs, Ryan E.en
dc.contributor.committeeMemberJiang, Youxingen
dc.creatorNguyen, Nam Xuanen
dc.creator.orcid0000-0002-3379-5765
dc.date.accessioned2020-06-02T21:06:57Z
dc.date.available2020-06-02T21:06:57Z
dc.date.created2018-05
dc.date.issued2018-04-13
dc.date.submittedMay 2018
dc.date.updated2020-06-02T21:06:57Z
dc.description.abstractThe mitochondrial calcium uniporter is a highly selective calcium channel localized to the inner mitochondrial membrane. Here, I describe the structure of an MCU ortholog from the fungus Neosartorya fischerii (NfMCU) determined to 3.8 Å resolution by phase-plate cryo-electron microscopy. The channel is a homotetramer with two-fold symmetry in its amino terminal domain (NTD) that adopts a similar structure to that of human MCU. The NTD assembles as a dimer of dimer to form a tetrameric ring that connects to the transmembrane domain through an elongated coiled-coil domain. The ion conducting pore domain maintains four-fold symmetry with the selectivity filter positioned at the start of the pore forming TM2 helix. The aspartate and glutamate sidechains of the conserved DIME motif are oriented toward the central axis and separated by one helical turn. Thus, the structure of NfMCU offers new insights into channel assembly, selective calcium permeation, and inhibitor binding.en
dc.format.mimetypeapplication/pdfen
dc.identifier.oclc1156324533
dc.identifier.urihttps://hdl.handle.net/2152.5/8316
dc.language.isoenen
dc.subjectCalcium Channelsen
dc.subjectCryoelectron Microscopyen
dc.subjectNeosartoryaen
dc.titleStructural Basis of Calcium Transport and Selectivity in the Mitochondrial Calcium Uniporteren
dc.typeThesisen
dc.type.materialtexten
thesis.degree.departmentGraduate School of Biomedical Sciencesen
thesis.degree.disciplineMolecular Biophysicsen
thesis.degree.grantorUT Southwestern Medical Centeren
thesis.degree.levelDoctoralen
thesis.degree.nameDoctor of Philosophyen

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
NGUYEN-DISSERTATION-2018.pdf
Size:
32.2 MB
Format:
Adobe Portable Document Format
Download

License bundle

Now showing 1 - 1 of 1
No Thumbnail Available
Name:
LICENSE.txt
Size:
1.84 KB
Format:
Plain Text
Description:
Download

Collections

UT Southwestern Graduate School of Biomedical Sciences