Identification of Drivers of Tumor Lymphangiogenesis in Non-Small Cell Lung Carcinoma (NSCLC)

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dc.contributor.otherDellinger, Michaelen
dc.contributor.otherBrekken, Rolfen
dc.creatorSibley, Robert Carsonen
dc.date.accessioned2015-07-14T12:56:02Z
dc.date.available2015-07-14T12:56:02Z
dc.date.issued2014-02-04
dc.descriptionThe 52nd Annual Medical Student Research Forum at UT Southwestern Medical Center (Tuesday, February 4, 2014, 3-6 p.m., D1.502)en
dc.descriptionEach year the Medical Student Research Program awards students for the best oral presentation and the best poster presentation as judged by faculty across campus. This author received an award as one of the best poster presentations at this forum.en
dc.description.abstractBACKGROUND: Non-small Cell Lung Carcinomas (NSCLCs) frequently spread to regional lymph nodes before they colonize other regions of the body, and the status of regional lymph nodes is an important prognostic factor for predicting the outcome of patients with lung cancer. It has recently been demonstrated that lymphangiogenesis, the sprouting of new lymphatic vessels from pre-existing vessels, facilitates the lymphogenous dissemination of NSCLC. However, the molecular mechanisms driving lymphangiogenesis in NSCLC are poorly understood. Objective: Our aim was to identify novel lymphangiogenic genes by identifying lymphangiogenic lung tumor cell lines, and then to use microarray data to generate a "lymphangiogenic" gene signature. METHODS: Tumors from 13 lung tumor cell lines were stained with antibodies against LYVE-1 and Podoplanin. Lymphatic vessels were counted in 5 representative 20X fields per tumor. Average lymphatic vessel densities were then calculated. Cell lines were grouped into lymphangiogenic, non-lymphangiogenic, and intermediate categories. Microarray data from the two extreme groups were then compared to generate a "lymphangiogenic" signature. RESULTS: Four cell lines, (Calu-1, H1993, HCC461, and HCC827) displayed high intratumoral lymphatic density, and five cell lines (Calu-3, H1155, H1395, H1975, and H2073) displayed no intratumoral lymphatic vessels. The "lymphangiogenic" signature obtained from the microarray data from these groups contained 146 genes, including the lymphatic growth factor VEGF-C. CONCLUSIONS: Our preliminary findings suggest that VEGF-C is an important driver of tumor lymphangiogenesis in NSCLC. The other 145 genes in the signature may also serve novel functions in regulating tumor lymphangiogenesis. Together, the results from this project provide mechanistic insight into the process of tumor lymphangiogenesis and metastasis. We believe that this information will lead to the development of new prognostic or predictive markers and therapeutic strategies to improve the outcome of patients with lung cancer.en
dc.description.sponsorshipSouthwestern Medical Foundationen
dc.identifier.citationSibley, R. C., Dellinger, M. & Brekken, R. (2014, February 4). Identification of drivers of tumor lymphangiogenesis in non-small cell lung carcinoma (NSCLC). Poster session presented at the 52nd Annual Medical Student Research Forum, Dallas, TX. Retrieved from https://hdl.handle.net/2152.5/1689en
dc.identifier.urihttps://hdl.handle.net/2152.5/1689
dc.language.isoenen
dc.relation.ispartofseries52nd Annual Medical Student Research Forumen
dc.subjectClinical Research and Case Reportsen
dc.subject.localBest Poster Presentation Awarden
dc.subject.meshCarcinoma, Non-Small-Cell Lungen
dc.subject.meshVascular Endothelial Growth Factor Cen
dc.subject.meshLung Neoplasmsen
dc.subject.meshLymphangiogenesisen
dc.subject.meshLymph Nodesen
dc.titleIdentification of Drivers of Tumor Lymphangiogenesis in Non-Small Cell Lung Carcinoma (NSCLC)en
dc.typePresentationen

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