Novel Activities of Kinase-Fold Enzymes from Legionella pneumophila

dc.contributor.advisorCobb, Melanie H.en
dc.contributor.committeeMemberTagliabracci, Vincent S.en
dc.contributor.committeeMemberMendell, Joshua T.en
dc.contributor.committeeMemberOlson, Eric N.en
dc.creatorBlack, Milesen
dc.creator.orcid0000-0002-2864-1710 2020
dc.description.abstractProtein kinases are fundamental mediators of cell signaling that transfer phosphate from ATP to their substrates. The protein kinase superfamily encompasses a vast and diverse trove of enzymes from all domains of life, including remote members that are barely recognizable by their primary amino acid sequence. SidJ (Substrate of Icm/Dot J) is a distant protein kinase homolog from the human pathogen Legionella pneumophila. Contamination of water supplies with Legionella bacteria is a frequent cause of deadly pneumonia outbreaks (Legionnaire's disease). SidJ is a secreted Legionella virulence factor required for bacterial intracellular replication, but it is unknown how SidJ contributes to pathogenesis of Legionnaire's disease, or if SidJ has maintained the kinase fold or catalytic activity. In this work, I determine that SidJ is a calmodulin-binding protein which adopts a protein kinase fold. However, instead of phosphorylation, it catalyzes protein polyglutamylation. SidJ utilizes ATP to form an isopeptide bond between the amino group of free glutamate and the 𝛾-carboxyl group of a glutamate of its substrate. During infection, SidJ polyglutamylates and inactivates a family of Legionella "all-in-one" ubiquitin ligases. Polyglutamylation is crucial step in the intracellular lifecycle of the bacterium and is required for full Legionella virulence in a eukaryotic host. SidJ reveals the unexpected catalytic versatility of the protein kinase fold, and highlights a unique strategy that pathogenic bacteria use to thrive within host cells. Interestingly, SidJ lacks key catalytic residues believed to be required for kinase activity. The discovery that SidJ is a polyglutamylating enzyme suggests that catalytically incompetent or 'pseudo' enzymes may lack activity only when assayed for the wrong reaction.en
dc.subjectBacterial Proteinsen
dc.subjectLegionella pneumophilaen
dc.subjectPolyglutamic Aciden
dc.subjectProtein Kinasesen
dc.subjectUbiquitin-Protein Ligasesen
dc.subjectVirulence Factorsen
dc.titleNovel Activities of Kinase-Fold Enzymes from Legionella pneumophilaen
dc.type.materialtexten School of Biomedical Sciencesen Regulationen Southwestern Medical Centeren of Philosophyen


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