Quantitative Image Analysis in the Study of Neutrophil and Neutrophil-Like HL-60 Chemotaxis

Simple item page
dc.contributor.advisorTu, Benjaminen
dc.contributor.committeeMemberCobb, Melanie H.en
dc.contributor.committeeMemberAltschuler, Steven J.en
dc.contributor.committeeMemberWu, Lanien
dc.contributor.committeeMemberStull, Jamesen
dc.contributor.committeeMemberBlount, Paulen
dc.creatorZhang-Velten, Elizabeth Renen
dc.creator.orcid0000-0003-0912-0351
dc.date.accessioned2019-06-03T19:56:48Z
dc.date.available2019-06-03T19:56:48Z
dc.date.created2017-05
dc.date.issued2017-04-11
dc.date.submittedMay 2017
dc.date.updated2019-06-03T19:56:49Z
dc.description.abstractNeutrophils are fast-moving first responders of the innate immune system. External chemoattractant signals result in neutrophil polarization: the neutrophil forms a leading edge (front) which constantly protrudes and retracts actin-rich pseudopods, and a contractile myosin-enriched trailing edge (back) which is insensitive and directionally persistent. Previous work has suggested that polymerized actin and contractile actomyosin segregate to the neutrophil's morphological front and back, respectively, due to mutual inhibition. Beyond this initial establishment of spatially segregated domains, however, many questions remain unclear. In this work, I address two questions: (i) first, how do the front and back of the neutrophil demonstrate seemingly uncoupled behaviors despite these inhibitory links? (ii) and, second, at what fMLP concentration does neutrophil chemotaxis saturate, and how is this maximal concentration determined? With quantitative analysis of immunofluorescent fixed-cell images and live-cell migration videos, I demonstrate the role of microtubules in insulating the front and back modules of chemotactic neutrophils, and the role of ERK in driving neutrophil migration into maximal fMLP concentrations.en
dc.format.mimetypeapplication/pdfen
dc.identifier.oclc1103324390
dc.identifier.urihttps://hdl.handle.net/2152.5/6614
dc.language.isoenen
dc.subjectCell Movementen
dc.subjectChemotaxisen
dc.subjectHL-60 Cellsen
dc.subjectNeutrophilsen
dc.titleQuantitative Image Analysis in the Study of Neutrophil and Neutrophil-Like HL-60 Chemotaxisen
dc.typeThesisen
dc.type.materialtexten
thesis.degree.departmentGraduate School of Biomedical Sciencesen
thesis.degree.disciplineIntegrative Biologyen
thesis.degree.grantorUT Southwestern Medical Centeren
thesis.degree.levelDoctoralen
thesis.degree.nameDoctor of Philosophyen

Files

Original bundle

Now showing 1 - 1 of 1
Thumbnail Image
Name:
ZHANG-VELTEN-DISSERTATION-2017.pdf
Size:
3.4 MB
Format:
Adobe Portable Document Format
Download

License bundle

Now showing 1 - 1 of 1
No Thumbnail Available
Name:
LICENSE.txt
Size:
1.85 KB
Format:
Plain Text
Description:
Download

Collections

UT Southwestern Graduate School of Biomedical Sciences